Dr. rer. nat. Daniel Wesner
Contact:
Dr. rer. nat. Daniel Wesner
University of Siegen
Physical Chemistry 1
AR-F0204
Adolf-Reichwein-Str. 2
57076 Siegen
Phone: ++49 (0)271 / 740 - 3841
Fax: ++49 (0)271 / 740 - 2805
email: wesner(at)chemie.uni-siegen.de
Research interests
Surface chemistry and characterization
Cell culture
Microscopy (AFM, SEM, Confocal Microscopy)
Project: Polymer coatings for cell separation and purification
We develop functional polymer coatings that control cell adhesion on surfaces used for cell culture. The release of cells is initiated by a thermal trigger that reversibly switches the polymer from cell binding to a water swollen coil (polymer brush) preventing cell adhesion. We work on the separation and purification of different cell types by optimizing the properties of the polymer layer for the characteristics of individual cells. As an example we realized the separation of induced pluripotent stem cells (iPS) from differentiated cells which is important for the purification in stem cells applications. Our current focus lies on broadening the range of cells so that these coatings can be used as a platform technology in the context of stem cell technology, tissue derived primary cells and biopsies.